Biochemistry 9th Edition Campbell – Test Bank

1. Which of following statements describes a difference between replication of DNA and transcription of RNA?
a. Nucleoside triphosphates are the precursors for replication, but nucleoside diphosphates are used for transcription.
b. Both strands of DNA are copied in replication, but usually only one is copied in transcription.
c. Base pairing is used to copy the sequence in replication, but not in transcription.
d. The chain grows from the 5′ to the 3′ end in replication, but 3′ to 5′ in transcription.
e. None of these
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Transcription Overview
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

2. Which of following statements concerning RNA transcription is false?
a. The DNA strands become separated during synthesis.
b. Synthesis of RNA is as accurate as synthesis of DNA
c. The template strand is read in the 3′ → 5′ direction.
d. All 4 ribonucleotides are required.
e. A primer is required for RNA synthesis
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Transcription Overview
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

3. Which of following statements concerning RNA transcription is false?
a. The release of pyrophosphate from a nucleoside triphosphate drives the reaction.
b. RNA is synthesized from the 5′ end to the 3′ end.
c. DNA to RNA base pairing includes A to U and G to C.
d. Transcription requires the use of a primer.
e. None of these
ANSWER: d
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Transcription Overview
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

4. The enzyme principally responsible for RNA synthesis in Escherichia coli
a. is a multisubunit enzyme
b. consists of a single polypeptide chain
c. requires Mn2+ for activity
d. requires a DNA primer
ANSWER: a
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

5. The end of the new mRNA molecule in E. coli. usually terminates in a string of A’s
a. True
b. False
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

6. Which of the following correctly describes a difference between RNA & DNA polymerases?
a. RNA polymerases usually do not need a template, while DNA polymerases do.
b. DNA polymerases usually require a primer (i.e., they can only continue a strand, not start one), while most RNA polymerases do not.
c. RNA polymerases usually synthesize introns, while DNA polymerases synthesize cistrons.
d. RNA polymerases polymerize 5′ → 3′, while DNA polymerases polymerize 3′ → 5′.
e. None of these
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

7. How do the core enzyme and the holoenzyme of RNA polymerase differ in E. coli?
a. The holoenzyme includes the sigma (σ) subunit, the core enzyme does not.
b. The core enzyme includes the sigma (σ) subunit, the holoenzyme does not.
c. The holoenzyme transcribes from an RNA template, the core enzyme from a DNA template.
d. The core enzyme transcribes from an RNA template, the holoenzyme from a DNA template.
ANSWER: a
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.1 – New in 6e
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

8. Which of these terms does not describe the DNA strand used to direct RNA synthesis?
a. Template strand
b. Coding strand
c. Antisense strand
d. Negative or “−” strand
e. All these terms describe the DNA strand used to direct RNA synthesis
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

9. At what point does the sigma (σ) subunit of RNA polymerase released from the core enzyme?
a. Prior to the incorporation of any nucleotides into an RNA strand.
b. After transcription begins and about 10 nucleotides have been added to the RNA chain.
c. Just prior to chain termination.
d. Never; it is an intrinsic part of the core enzyme.
e. After RNA polymerase discovers an inverted repeat
ANSWER: b
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.7 – Modified in 8e
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM

10. RNA synthesis begins at the base in the DNA sequence designated by the following number:
a. +1 (plus one)
b. 0 (zero)
c. −1 (minus one)
d. −10 region (minus ten)
e. It varies between genes.
ANSWER: a
POINTS: 1
QUESTION TYPE: Multiple Choice
HAS VARIABLES: False
LEARNING OBJECTIVES: CAFA.BIOC.15.5 – Modified from 5e
TOPICS: Prokaryotic Transcription
DATE CREATED: 12/23/2013 2:14 PM
DATE MODIFIED: 12/23/2013 2:14 PM