Molecular Diagnostics Fundamentals Methods and Clinical Applications 1st Edition by Lela Buckingham -Test Bank

1. Which of the following polymorphisms are single nucleotide differences that are seen between two genomes?
A) Restriction fragment length polymorphisms
B) Short tandem repeats
C) Variable number tandem repeats
D) Single nucleotide polymorphisms

2. Southern blot is used to detect which of the following polymorphisms?
A) Short tandem repeats
B) Single nucleotide polymorphisms
C) Restriction fragment length polymorphisms
D) Long interspersed nucleotide sequences

3. If a locus is homozygous, then the locus has:
A) many different alleles
B) two alleles that are the same
C) two alleles that are different
D) no alleles

4. DNA was isolated from a patient and cut with HaeIII. The resulting fragments were separated by gel electrophoresis and transferred to a membrane. The fragments were allowed to hybridize to a probe, and the resulting band pattern was compared with that seen in another sample of DNA. Which of the following methods is described?
A) RFLP by Southern blotting
B) VNTR by PCR
C) SNP by sequencing
D) STR by PCR

5. FGA, D8S1179, and D2S11 are examples of which of the following?
A) RFLP
B) VNTR
C) STR
D) SNP

6. Which of the following is used as a marker of gender?
A) D8S1179
B) Amelogenin
C) TPOX
D) PentaD

7. How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?
A) 10
B) 12
C) 13
D) 16

8. When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?
A) 1
B) 2
C) 4
D) 6

9. Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?
A) STR
B) Y-STR
C) RFLP
D) VNTR

10. Which of the following STRs is a Y-STR?
A) D5S818
B) vWA
C) Penta E
D) DYS385

Answer Key

1. D
2. C
3. B
4. A
5. C
6. B
7. C
8. A
9. B
10. D