Test Bank Brock Biology Of Microorganisms 14th Edition By Michael T. Madigan

Brock Biology of Microorganisms, 14e (Madigan et al.)

Chapter 11  Genetic Engineering and Biotechnology

Multiple Choice Questions

1) If a foreign gene is cloned into an expression host, it is important that the host itself

1. A) not produce the protein being studied.
2. B) produce the protein in larger amounts than the vector.
3. C) repress the genetic expression being studied.
4. D) produce signal proteins to tag the host protein.

Answer:  A

Bloom’s Taxonomy:  Comprehension

Chapter Section:  11.9

2) Detecting a specific protein with an antibody is considered a(n) ________ method.

1. A) selection
2. B) screening
3. C) isolation
4. D) duplication

Answer:  B

Bloom’s Taxonomy:  Knowledge

Chapter Section:  11.4

3) To discover a catabolic gene cluster, cloning large sequences of approximately 40 kbp requires the utility of

1. A) bacterial artificial chromosomes (BACs).
2. B) cosmids or fosmids.
3. C) a eukaryotic host to house the large foreign DNA.
4. D) multiple gene fusions.

Answer:  B

Bloom’s Taxonomy:  Application

Chapter Section:  11.10

4) Expression vectors are designed to ensure that ________ can be efficiently ________.

1. A) mRNA / transcribed
2. B) DNA / transcribed
3. C) mRNA / translated
4. D) DNA / translated

Answer:  A

Bloom’s Taxonomy:  Comprehension

Chapter Section:  11.9

5) A(n) ________ gene is a gene that encodes a protein that is easy to detect and assay.

1. A) encoder
2. B) translational
3. C) reporter
4. D) recorder

Answer:  C

Bloom’s Taxonomy:  Knowledge

Chapter Section:  11.6

6) One of the more formidable obstacles to mammalian gene cloning is the presence of

1. A) introns.
2. B) exons.
3. C) repressors.
4. D) integrators.

Answer:  A

Bloom’s Taxonomy:  Knowledge

Chapter Section:  11.11

7) Which of the following is NOT a characteristic of a type II restriction endonuclease?

1. A) cleavage product can be either blunt or sticky ended but is always the same for an individual enzyme
2. B) recognizes a specific palindromic site for cleavage
3. C) recognition site length varies among enzymes but is always the same for an individual enzyme
4. D) unable to cleave methylated DNA

Answer:  D

Bloom’s Taxonomy:  Comprehension

Chapter Section:  11.1

8) What type of vector can replicate and be maintained stably in two (or more) unrelated host organisms?

1. A) virus
2. B) expression
3. C) shuttle
4. D) integrating

Answer:  C

Bloom’s Taxonomy:  Knowledge

Chapter Section:  11.9

9) The genes encoding luciferase, green fluorescent protein (GFP), and β-galactosidase are typically used in cloning as

1. A) transcription regulators.
2. B) global control genes.
3. C) promoter sequences.
4. D) reporter genes.

Answer:  D

Bloom’s Taxonomy:  Application

Chapter Section:  11.6

10) To verify a gene was cloned into a vector successfully, sequencing the vector as well as ________ are commonly performed.

1. A) agarose gel electrophoresis
2. B) fluorescence in situ hybridization
3. C) protein purification
4. D) northern blots

Answer:  A

Bloom’s Taxonomy:  Application

Chapter Section:  11.1

11) Which objective would be best to use a Southern blot rather than a Northern blot?

1. A) Determine if a gene is present in a genome.
2. B) Discover gene function.
3. C) Identify regulatory gene-protein interactions.
4. D) Quantify expression profiles of a gene.

Answer:  A

Bloom’s Taxonomy:  Application

Chapter Section:  11.2